QuEChERS Solutions

Even Easier

Comprehensive Solutions Simplify
Sample Prep and Analysis

  • New free-flowing salts in slim packs make extraction a snap.
  • Complete line of easy-to-use QuEChERS products, reference standards, and accessories.
  • Follow up sample prep with a wide range of analytical columns for both LC and GC.

Keep your workflow running smoothly with Q-sep extraction salts from Restek!

Pure Satisfaction
Pure Satisfaction

For years, the QuEChERS approach to sample preparation has been making the lives of food safety scientists easier. Gone are the days of time-consuming, solvent-intensive extraction techniques and multiple solid phase extraction cartridge cleanup steps! Research published by the U.S. Department of Agriculture Easter Regional Research Center in Wyndmoor, PA, [1] introduced to the world QuEChERS—a method that is Quick, Easy, Cheap, Effective, Rugged, and Safe.

With QuEChERS, a homogenized sample undergoes a quick extraction step where the analytes are driven into an organic solvent by the partitioning power of a blend of salts. After extraction, the sample is cleaned up through the use of a dispersive solid phase extraction (dSPE) step that is also quick and easy to perform. This simple, two-stage process offers significant savings in time, materials, and effort, making QuEChERS sample preparation faster and easier than other approaches.

Table I: Prepare samples more quickly, easily, and cost effectively with QuEChERS.


Mini-Like or Modified
Luke Method


with QuEChERS

Estimated time to process 6 samples (min)



4x faster

Solvent used (mL)



6-9x less solvent

Chlorinated waste (mL)



Safer, cheaper, greener

Glassware/specialized equipment

capacity for 200 mL, quartz wool,
funnel, water bath, or evaporator



Since its introduction, the QuEChERS technique has evolved to accommodate an expanding list of pesticides in an increasingly diverse list of foodstuffs. At Restek, we offer Q-sep products to cover the four major approaches to QuEChERS, along with a host of other items to help make your QuEChERS experience simple and successful. We carry a comprehensive line of sample prep supplies, reference standards, and LC and GC columns that will help make QuEChERS even easier, whether you are new to the approach or developing a method for a new sample matrix.

Which product do I choose?

QuEChERS is primarily a two-stage process, and you’ll select Q-sep products for both stages. Your method may have specific recommendations, but in case you’re not sure what will work, here some considerations when making your selection.

Stage 1: Extraction

The choice of which extraction technique to use is made principally by considering your analytes of interest. If you’re looking for compounds that aren’t pH sensitive, the original unbuffered method [1] will work great. However, if your analytes of interest are pH sensitive, you will want to select one of the buffered methods—notably, the official EN 15662 method [2], the mini-multiresidue approach [3], or the official AOAC 2007.01 method [4].

Restek’s Q-sep products for the two European approaches, the official EN 15662 method and the mini-multiresidue approach, are the same, so it becomes essentially a choice between a European-developed method and the AOAC method. Both are buffered to lower the pH to a range where most pesticides are stable, and the methods differ only in the specific buffering salts and their ratios. Either would be a good place to start for most analyses. There are a great many studies that highlight specific instances when one buffering technique may outperform another, so a little research into your particular area of study may yield practical suggestions on which extraction method to use.

Stage 2: Dispersive Solid Phase Extraction (dSPE) Cleanup

The next big choice comes during the selection of which dSPE cleanup product to use. Where the choice of the extraction product was driven largely by the analytes of interest, the choice of cleanup is based on the sample being tested. Restek Q-sep dSPE products are formulated with different sorbents in different ratios so that your dSPE choice can be tailored to the composition of your particular sample type (e.g., fatty, highly pigmented, etc.). Use Table II to learn what each sorbent removes and to help select the best dSPE product for your particular sample.

Table II: Visit the desktop version of to access this table.

Table II: Select your dSPE tube based on sample type to ensure you get the best sorbent blend for removing specific matrix interferences.

Method Sorbent Mass (mg)
MgSO4 PSA* C18-EC GCB** Vial Volume (ml)
Excess Water Sugars, fatty acids, organic acids, anthocyanine pigments Lipids, nonpolar interferences Pigments, sterols, nonpolar interferences
lettuce General fruits and vegetables (e.g., celery, head lettuce, cucumber, melon) AOAC 2007.01 150 50 -
- 2 26124
Original unbuffered, EN 15662, mini-multiresidue 150 25 - - 2 26215
AOAC 2007.01 1,200 400 - - 15 26220
Original unbuffered, EN 15662 900 150 - - 15 26223
avocado Foods with fats and waxes (e.g., cereals, avocado, nuts, seeds, and dairy) Mini-multiresidue 150 25 25 - 2 26216
- 150 - 50 - 2 26242
AOAC 2007.01 150 50 50 - 2 26125
AOAC 2007.01 1,200 400 400 - 15 26221
- 1,200 - 400 - 15 26244
- 900 150 150 - 15 26226
strawberries Pigmented fruits and vegetables (e.g., strawberries, sweet potatoes, tomatoes) Mini-multiresidue, EN 15662 150 25 - 2.5 2 26217
AOAC 2007.01 150 50 - 50
2 26123
AOAC 2007.01 1,200
400 400 400 15 26222
EN 15662 900 150 - 15 15 26224
General Purpose Highly pigmented fruits and vegetables (e.g., red peppers, spinach, blueberries) Mini-multiresidue, EN 15662 150 25 - 7.5 2 26218
AOAC 2007.01 150 50 50 50 2 26219
EN 15662 900 150 - 45 15 26225
- 900 300 - 150 15 26126
General Purpose General purpose (wide range of commodities, including fatty and pigmented fruits and vegetables) - 150 50 50 7.5 2 26243
- 900 300 300 45 15 26245

Note: No entry in the Method column refers to dSPE formulations not specifically included in one of the cited references. These products can be used to accommodate the various needs of specific matrices not directly met by the cited references.
*PSA = primary secondary amine exchange material
**GCB = graphitized carbon black

Restek is not able to provide copies of these documents.
[1] M. Anastassiades, S.J. Lehotay, D. Stajnbaher, F.J. Schenck, Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solid-phase extraction" for the determination of pesticide residues in produce. J. AOAC Int. 86 (2003) 412-431.
[2] EN 15662, Foods of Plant Origin—Determination of Pesticide Residues Using GC-MS and/or LC-MS/MS Following Acetonitrile Extraction/Partitioning and Clean-up by Dispersive SPE—QuEChERS method, 2008.
[3] QuEChERS-A Mini-Multiresidue Method for the Analysis of Pesticide Residues in Low-Fat Products, 2004.
[4] AOAC Official Method 2007.01, Pesticide Residues in Foods by Acetonitrile Extraction and Partitioning with Magnesium Sulfate, 2007.


Colonnes LC “core-shell” Raptor ARC-18 (USP L1)

  • Idéale pour des analyses LC-MS/MS rapides et avec une préparation d’échantillon sommaire.
  • Profil de rétention bien équilibré pour une meilleure détection et quantification d’une multitude de composés les plus variés.
  • Stériquement protégée pour résister aux phases mobiles de faible pH et garantir une rétention constante.
  • Une colonne de la gamme Raptor Restek (à base de particules « core-shell »)
  • Certificat d’analyse spécifique au numéro de série téléchargeable en ligne.

Colonnes Rxi-5Sil MS (silice fondue)

(phase de faible polarité; Crossbond 1,4-bis(diméthylesiloxy)phénylène diméthyle polysiloxane)

  • Spécialement conçue pour un « bleeding » ultra faible en GC/MS.
  • Colonnes pour applications générales : analyse GC/MS des hydrocarbures chlorés, des phtalates, des phénols, des amines, des pesticides organochlorés, des pesticides organophosphorés, des stupéfiants, des impuretés dans les solvants et des hydrocarbures. Excellente inertie vis-à-vis des composés actifs.
  • Températures limites d’utilisation : -60°C à 350°C.

Reference Standards

QuEChERS Internal Standards and Quality Control Standards

  • Single and multiple component standards as specified in EN Method 15662.
  • Support for GC and HPLC with MS, MS/MS, and selective detectors.
  • Ready to use for QuEChERS extractions—no dilutions necessary.

Multi-résidus de pesticides pour LC-MS/MS (204 pesticides dans un kit de 10 solutions-étalons)

  • Idéals pour une quantification précise de pesticides couramment recherchés dans les matrices alimentaires ou environnementales.
  • 204 pesticides répartis en 10 solutions-étalons dont la composition garantit une parfaite stabilité de chaque composé (solutions-étalons également proposées individuellement).
  • Chaque solution-étalon est testée pour en vérifier la composition. Un certificat d’analyse complet est systématiquement fourni.
  • Méthode d’analyse de multi-résidus de pesticides optimisée fournie.

Multi-résidus de pesticides pour GC-MS/MS (203 pesticides dans un kit de 9 solutions-étalons)

  • Idéals pour une quantification précise de pesticides couramment recherchés dans les matrices alimentaires ou environnementales.
  • 203 pesticides répartis en 9 solutions-étalons dont la composition garantit une parfaite stabilité de chaque composé (solutions-étalons également proposées individuellement).
  • Chaque solution-étalon est testée pour en vérifier la composition. Un certificat d’analyse complet est systématiquement fourni.
  • Méthode d’analyse de multi-résidus de pesticides optimisée fournie.
  • Documentation technique
    Étalons certifiés pour l’analyse de 380 pesticides par LC-MS/MS et GC-MS/MS

Sample Handling

Etuis QuEChERS Q-Sep

  • Quantités pré dosées. Qualité ultra-pure.
  • Etuis étroits pouvant être introduits dans les tubes pour un transfert facile.
  • Tubes prêts à l’emploi.
  • Recommandées pour les méthodes QuEChERS AOAC (2007.1) et EN 15662.

Tubes QuEChERS Q-sep pour purification d'extraits par dSPE

Préparation rapide et simple des échantillons avec les tubes QuEChERS Q-sep RESTEK

  • Conditionnés par 10 dans des emballages protecteurs.

Centrifugeuse Q-sep 3000

pour tubes QuEChERS

  • Compatible avec les tubes pour centrifugation de 2 ml, 15 ml et 50 ml.
  • Faible encombrement.
  • Sûre et fiable pour une utilisation intensive.

Distributeur Q-sep pour bouteille à solvant

  • 56 volumes préréglés de 2.5 ml à 30 ml (par pas de 0.5 ml)—idéal pour les méthodes QuEChERS !
  • Filetage de 30 mm. 4 adaptateurs fournis (25 mm, 28 mm, 38 mm et 45 mm).
  • Calibré individuellement selon la norme ISO 8655 (certificat fourni). Peut être aussi recalibré par l'utilisateur.
  • Les matériaux de fabrication (PTFE, verre et polypropylène) garantissent une excellente compatibilité chimique avec tous les solvants courants. Autoclavable.
  • Système de sécurité intégré. Buse coupe-goutte.
  • Démontage facile pour un nettoyage en profondeur.

Racks pour tubes Q-sep pour centrifugation

  • Pour tubes de 2 ml, 15 ml et 50 ml.
  • Repérage alphanumérique.
  • Assemblage facile.

Accessoires et pieces détachées

Insert d'injection Topaz 4 mm "Gooseneck splitless"

pour GC Agilent avec injecteur "split/splitless"

Les inserts d’injection Topaz sont le fruit d’une technologie révolutionnaire qui leur confère une inertie exceptionnelle.

  • Désactivation – une inertie inégalée pour des résultats d’analyses de traces exacts et précis avec des limites de détection toujours plus basses.
  • Reproductibilité – des contrôles à chaque étape de la fabrication et des tests poussés garantissent une fiabilité et des performances inégalées quelle que soit la classe chimique des composés analysés.
  • Longévité – meilleure résistance aux injections répétées pour une fréquence de remplacement plus faible.
  • Satisfaction 100% garantie – si un insert d’injection Topaz ne semble pas conforme aux caractéristiques décrites ci-dessus, nous nous engageons à l’échanger ou à le rembourser*.


Evaluation of Dispersive and Cartridge Solid Phase Extraction (SPE) Cleanups for Multiresidue Pesticides in QuEChERS Extracts of Finished Tobacco Using GCxGC-TOFMS

We compared the efficacy of dSPE and cSPE cleanup methods for multiresidue pesticide analysis of QuEChERS extracts of finished tobacco products using GCxGC-TOFMS. Cleanup with dSPE tubes containing 7.5 mg of GCB and 25 mg of PSA produced better results than other methods, with average recoveries of 92% and 91% for 500 ppb and 50 ppb fortifications respectively.


Comparing Pesticide Residues in Amish and Commercially Grown Strawberries and Spinach Using QuEChERS, Various dSPE Sorbents, and GC-TOFMS

QuEChERS extraction, dSPE cleanup, and GC-TOFMS analysis were used to assess pesticide residue levels in strawberry and spinach from both commercial and Amish growers. Various dSPE sorbent products were compared to determine which were most effective.

PDF Document

Determining Pesticides in Dietary Supplements with QuEChERS Extraction, Cartridge SPE, and GCxGC-TOFMS

The novel approach used here combines QuEChERS extraction, cartridge SPE cleanup, and GCxGC-TOFMS analysis, resulting in good recoveries for a wide range of pesticides in dietary supplements. Matrices include dandelion root, sage, and a multi-herb finished product. (PDF - 0MB)

PDF Document

Mitigating Matrix Effects: Examination of Dilution, QuEChERS, and Calibration Strategies for LC-MS/MS Analysis of Pesticide Residues in Diverse Food Types

LC-MS/MS is popular for monitoring pesticide residues in food due to its selectivity and sensitivity; however, matrix effects can cause poor data quality and difficult quantification. We evaluated the relative effectiveness of sample dilution, QuEChERS cleanup, solvent-based calibration, and matrix-matched calibration strategies to mitigate matrix effects in celery, kale, avocado, lime, and brown rice flour. (PDF - 0MB)

PDF Document

QuEChERS Made Even Easier: Comprehensive Solutions Simplify Sample Prep and Analysis

Learn how to use the QuEChERS approach to perform faster, easier sample preparation and cleanup for multiresidue pesticide analysis. Q-sep QuEChERS products save time, money, and materials compared to modified Luke methods. (PDF - 0MB)

PDF Document

Developing New Methods for Pesticides in Dietary Supplements

QuEChERS is a simple, effective approach to sample prep that can be applied to the analysis of pesticides in dietary supplements. Here, we demonstrate a QuEChERS, cSPE, GC-TOFMS procedure that results in good recoveries for a wide range of pesticides in dandelion root. (PDF - 0MB)

PDF Document
PDF Document

Fast, Simple QuEChERS Extraction and Cleanup of Pesticide Residue Samples

QuEChERS dSPE methods simplify extract cleanup, reduce material costs, and improve sample throughput. Here we demonstrate the effectiveness of QuEChERS sample cleanup using a multiresidue analysis of pesticides on strawberries. (PDF - 0MB)

PDF Document

Fast Screening of Recalled Tylenol for Tribromoanisole and Related Adulterants

A recall of Tylenol pain reliever and other related products highlights the need for simple, quick sample preparation and a comprehensive analytical method for adulterants in consumer products. The rush to examine a multitude of samples in a short period of time is a common scenario for potential recalls, especially when a contaminant is found in a given product and rapid determinations need to be made to assess how widespread the problem may be. (PDF - 0MB)

PDF Document

How Column Inertness Improves the Chromatography of Basic Compounds

Not all column deactivations are appropriate for analyzing basic compounds. Here, we demonstrate the effect of column inertness on peak shape and discuss its role in improving method accuracy, sensitivity, and development time. (PDF - 0MB)

Audio Slideshow

Introduction to QuEChERS

A brief overview of the QuEChERS process, and how it can simplify your sample preparation workflow.

Multimedia On-Demand Webinars

Sample Preparation of Cannabinoids in Urine Using the QuEChERS Method and dSPE Cleanup

This webinar introduces QuEChERS and dSPE and discusses their application to sample prep for GC/MS analysis of cannabinoids in urine. Attendees will learn how to increase sample throughput with this simple approach.

Narrated by . Length: 16 minutes

Analysis of Veterinary Drug Residues using a Unique HPLC Biphenyl Stationary Phase

Analysis of veterinary drug residues in milk using a QuEChERS approach and biphenyl column is discussed. Better retention is achieved, compared to C18 or phenyl phases, improving separation of analytes and matrix components.

Narrated by . Length: 24 minutes