Innovative Chromatography Products
www.restek.fr
www.restek.fr
Benzodiazepines in Urine on Raptor™ Biphenyl by LC-MS/MS
| Peaks | tR (min) | Q1 | Q3 Quantifier | Q3 Qualifier | |
|---|---|---|---|---|---|
| 1. | 7-Aminoclonazepam | 0.86 | 286.0 | 222.2 | 250.3 |
| 2. | 7-Aminoflunitrazepam | 1.15 | 284.2 | 135.2 | 240.2 |
| 3. | Flurazepam | 1.29 | 388.1 | 315.1 | 288.2 |
| 4. | Midazolam | 1.41 | 326.1 | 291.2 | 244.2 |
| 5. | N-Desmethylflunitrazepam | 1.76 | 300.0 | 254.1 | 225.2 |
| 6. | Lorazepam | 1.79 | 321.0 | 275.1 | 302.9 |
| 7. | Oxazepam | 1.86 | 287.1 | 241.0 | 104.1 |
| 8. | Clonazepam | 1.88 | 315.9 | 270.2 | 214.1 |
| 9. | Nitrazepam | 1.88 | 282.1 | 236.1 | 207.2 |
| 10. | Desalkylflurazepam | 1.93 | 289.0 | 140.1 | 165.1 |
| 11. | 2-Hydroxyethylflurazepam | 2.00 | 333.1 | 246.1 | 166.0 |
| 12. | Nordiazepam | 2.07 | 271.0 | 140.0 | 208.0 |
| 13. | Clobazam | 2.13 | 301.1 | 259.1 | 224.0 |
| 14. | Estazolam | 2.18 | 294.9 | 267.1 | 241.0 |
| 15. | Triazolam | 2.19 | 343.1 | 308.2 | 315.0 |
| 16. | Flunitrazepam | 2.19 | 314.4 | 239.1 | 211.0 |
| 17. | Temazepam | 2.23 | 301.2 | 255.0 | 177.1 |
| 18. | Alprazolam | 2.27 | 309.4 | 281.1 | 205.0 |
| 19. | Diazepam-d5 (IS) | 2.45 | 290.0 | 154.1 | - |
| 20. | Diazepam | 2.47 | 284.8 | 193.1 | 222.2 |
LC_CF0581
| Column | Raptor™ Biphenyl (cat.# 9309A5E) | ||||||||||||||||||||||||
| Dimensions: | 50 mm x 3.0 mm ID | ||||||||||||||||||||||||
| Particle Size: | 2.7 µm | ||||||||||||||||||||||||
| Guard Column: | Raptor™ Biphenyl EXP® 5 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253) | ||||||||||||||||||||||||
| Temp.: | 40 °C | ||||||||||||||||||||||||
| Sample | Benzodiazepine custom mix | ||||||||||||||||||||||||
| Conc.: | A 50 ng/mL standard was prepared in urine. 50 µL of 50 ng/mL urine standard was diluted with 950 µL of water prior to analysis (20x dilution). | ||||||||||||||||||||||||
| Inj. Vol.: | 20 µL | ||||||||||||||||||||||||
| Mobile Phase | |||||||||||||||||||||||||
| A: | 2 mM Ammonium formate and 0.2% formic acid in water | ||||||||||||||||||||||||
| B: | 0.2% Formic acid in methanol | ||||||||||||||||||||||||
| |||||||||||||||||||||||||
| Detector | ABSCIEX API 4000™ MS/MS | ||||||||||||||||||||||||
| Ion Source: | TurboIonSpray® | ||||||||||||||||||||||||
| Ion Mode: | ESI+ | ||||||||||||||||||||||||
| Mode: | Scheduled MRM | ||||||||||||||||||||||||
| Instrument | API LC-MS/MS |
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